CoRTecS

Detail

The core facility

Titan 1.jpg

Biologie cellulaire, Imagerie, Biologie moléculaire, Biochimie, Génomique, Biologie systémique, Développement, Biologie structurale Integrated Structural Biology Platform- FRISBI

  • Scientifical Manager Bruno KLAHOLZ
  • Technical Manager Catherine BIRCK
  • UMR 7104 / UMR_S 1258 - IGBMC / Institut de génétique et de biologie moléculaire et cellulaire
  • CNRS, INSERM, UNIVERSITE DE STRASBOURG
  • Ilkirch-Graffenstaden
  • Monosite
  • Service delivery
    • to the academic community
    • to the socio-economic world
  • Collaborations of research
    • with the academic community
    • to the socio-economic world
  • Affiliated institution of administrative supervision
    • CNRS
    • INSERM
    • UNIVERSITE DE STRASBOURG
BSI - FRISBI

The integrated structural biology platform of the center offers project-based access to all advanced tools from sample preparation (protein and macromolecular complexes) and biophysical characterization to structural analysis including in particular cryo-EM, X-ray crystallography, small angle X-ray scattering, super-resolution fluorescence microscopy and NMR of proteins and macromolecular complexes including nucleoprotein complexes. Taken together, this allows integrating functional data and various multi-resolution structural data.
The platform has also a training mission of the user on site and by the organisation of workshops.

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Expertises

Scientific expertise

  • 3D structure determination of proteins and macromolecular complexes
  • Protein production and purification
  • Biophysical characterisation of macromolecules
  • Crystallisation of macromolecules
  • Multi scale data integration

Technical expertise

  • Cryo-electron microscopy
  • X-ray crystallography
  • Protein production in mammalian cells and insect/ baculovirus systems
  • Protein purification
  • Crystallisation
  • Analytical ultracentrifugation
  • Binding affinity determination using BLI and ITC
  • Quality control of samples using mass photometry, SEC-MALS and nanoDSF
  • Super resolution microscopy
  • NMR

Resources

Main equipments

  • Cryo electron microscope Titan Krios G4 equipped with Falcon i4 direct electron detector and Selectris filter
  • Cryo electron microscope Glacios
  • Electron microscope Tecnai F20
  • Electron microscope cryo-FIB-SEM
  • NMR spectrometers 600MHz and 700MHz equipped with cryogenic probes
  • Cryo electron microscope Titan Krios equipped with K3/Falcon3 camera, GIF and Volta Phase Plate
  • Super-resolution microscope Leica SR GSD
  • Crystallisation robots Mosquito and visualisation systems Rock Imager
  • Analytical ultracentrifuge XL-I
  • BioLayer Interferometry Octet R8 instrument (BLI)
  • Vitrobot and Plasma Cleaner for EM grid preparation
  • Thermophoresis Monolith NT.115 system
  • NanoDSF Prometheus NT.48 system
  • Ultramicrotome and high pressure freezing system
  • TwoMP mass photometry instrument
  • PEAQ-ITC microcalorimeter

Specific rooms

  • Laboratoire L1
  • Laboratoire L2

Other kinds of rooms

  • Culture mammalian cell rooms L2
  • Culture insect/baculovirus cell rooms L2
  • Cold rooms for protein purification and crystalisation
  • Rooms stabilised at 20°C and 27°C for protein crystallisation
  • Culture mammalian cell room L1

Software resources / Data bases

  • Serial EM; EPU; Warp; CryoSparc;Topspin (Bruker); Autoproc; Buster; CCP4 suite; Chimera; Coot; Dials; HKL2000; HKL3000; Phenix Suite; Pymol; Shelx; Solve; XDS

Other kinds of resources

  • Bacterial strains, insect and mammalian cells
  • Procaryotic and eucaryotic expression vectors
  • Differtent types of columns for chromatography

Recent collaborative projects

RAR-scale

RAR-scale

Structural basis for DNA recognition and allosteric control of the retinoic acid receptors RAR-RXR (Osz et al., 2020)

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HiChemSynPro

HiChemSynPro

Development of new tools to set up protein library by chemical synthesis. The new approaches include de novo catalytical proteins and microfluidics reactors.

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Respi_complex

Respi_complex

Study by cryo-electron microscopy of a bacterial respiratory chain supercomplexes

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Core facility partners